Focused Efforts On High-throughput Processing Of FFPE Tissues For Proteomic Analysis: The utility of the Covaris R230 for LCM FFPE based proteomic projects
Benjamin Madden, Ph.D.
Thursday, June 8, 2023
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Chapters
Introduction
Proteomics Core
Our Introduction to Processing FFPE Samples
Continued Testing New Procedures
Manual Workflow
Must-haves for an Efficient, High-Throughput Workflow
Focused Ultrasonication Protein Solubilization
Adaptive Focused Acoustics
®
(AFA
®
) Technology
Laser Capture Microdissection (LCM)
Covaris AFA
®
Technology for Sample Processing and Protein Extraction from LCM/FFPE Samples
Evaluation: Processing Time
Mass Spectrometry Results
Optimization: Plate Format and Reproducibility (n = 16)
Accelerated with AFA
®
Yields the Highest Protein / Peptide Numbers
To Summarize the Initial Evaluation….
Simplify: Single Well Processing with No Transfers after Sample Collection: Proof of Principle
Protein IDs Across All Samples
Apply: Does AFA
®
have Relevance in Clinical Space?
Single Well Method Identifies the Correct Antigen!
Summary
Acknowledgement
Thank You
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