Immunoassays, particularly ELISAs, are becoming increasingly sophisticated through the use of advanced capture reagents and reporter systems.
Yet despite improved levels of sensitivity and specificity, it’s vital to remember the basics—the measured analyte should be fit-for-purpose, and the immunoassay designed to monitor it should be both robust and highly characterized.
Generally, cell proliferation assays measure the number of cells (or the change in the proportion of cells) that are dividing.
The main types, although differing in their nature of measurements, are adaptable to microplate readers and multiwell plates.
Moreover, they are available as convenient assay kits from multiple vendors, allowing researchers to take advantage of automation and higher-throughput technology.